Draft genome of the Klebsiella pneumoniae 24Kpn33 and complete sequence of its pCOL-1, a plasmid related to the blaKPC-2 acquisition in Pseudomonas aeruginosa

Deisy Abril; Duway Nicolas Lesmes-Leon; Ricaurte Alejandro Marquez-Ortiz; Aura Lucía Leal; Catalina Tovar-Acero; Zayda Lorena Corredor Rozo; Natasha Vanegas Gómez; Javier Escobar-Perez

doi:10.1128/mra.00071-24

Draft genome of the Klebsiella pneumoniae 24Kpn33 and complete sequence of its pCOL-1, a plasmid related to the bla_KPC-2 acquisition in Pseudomonas aeruginosa

Microbiol Resour Announc. 2024 Apr 11;13(4):e0007124. doi: 10.1128/mra.00071-24. Epub 2024 Mar 18.

Authors

Affiliations

¹ Bacterial Molecular Genetics Laboratory - LGMB, Universidad El Bosque, Bogotá, Colombia.
² University of Kaiserslautern-Landau (RPTU), Kaiserslautern, Germany.
³ German Research Center for Artificial Intelligence (DFKI), Kaiserslautern, Germany.
⁴ Facultad de Medicina y Grupo de Investigación en Enfermedades Infecciosas, Universidad Nacional de Colombia, Bogotá, Colombia.
⁵ Departamento de Patología y Laboratorios, Fundación Santa Fe de Bogotá, Bogotá, Colombia.
⁶ Grupo de Investigación en Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Montería, Colombia.
⁷ The i3 Institute, Faculty of Science, University of Technology, Sydney, Australia.

Abstract

We report the draft genome of a clinical multi-resistant Klebsiella pneumoniae (24Kpn33) isolate, whose genome (5.7 Mbp) harbored 17 antibiotic resistance genes, including bla_KPC-2. Notably, this gene was mobilized within the IncP-6 pCOL-1 plasmid, the first genetic platform related to the acquisition and dissemination of the bla_KPC-2 in Pseudomonas aeruginosa.

Keywords: Tn4401b; blaKPC-2; interspecies dissemination; pCOL-1.

Abstract

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