Preparation of Tau Condensates by Liquid-Liquid Phase Separation to Study Tau Amyloid Aggregation

Yanxian Lin

doi:10.1007/978-1-0716-3629-9_10

Preparation of Tau Condensates by Liquid-Liquid Phase Separation to Study Tau Amyloid Aggregation

Methods Mol Biol. 2024:2754:185-192. doi: 10.1007/978-1-0716-3629-9_10.

Author

Yanxian Lin¹

Affiliation

¹ Department of Biomolecular Science and Engineering, University of California, Santa Barbara, CA, USA.

PMID: 38512667
DOI: 10.1007/978-1-0716-3629-9_10

Abstract

Protein liquid-liquid phase separation (LLPS) has been associated with protein amyloid aggregation. Amyloid aggregation of tau is a hallmark of Alzheimer's disease and other neurodegenerative diseases. This protocol provides steps to prepare tau condensates via LLPS, so that researchers can further study its driving forces and its relationship with tau amyloid aggregation.

Keywords: Amyloid aggregation; Complex coacervation; Liquid–liquid phase separation; Tau proteinTauprotein.

MeSH terms

Alzheimer Disease* / metabolism
Amyloid / metabolism
Amyloidogenic Proteins
Humans
Phase Separation
tau Proteins* / metabolism

Substances

tau Proteins
Amyloid
Amyloidogenic Proteins