Generation of two isogenic iPSC lines from a healthy male donor of European ancestry

S Edwards; L Hagenau; B Nowack; J Rhode; M F Hossain; A Tzvetkova; L R Jensen; A W Kuss

doi:10.1016/j.scr.2024.103403

Generation of two isogenic iPSC lines from a healthy male donor of European ancestry

Stem Cell Res. 2024 Jun:77:103403. doi: 10.1016/j.scr.2024.103403. Epub 2024 Mar 24.

Authors

S Edwards¹, L Hagenau¹, B Nowack², J Rhode¹, M F Hossain¹, A Tzvetkova³, L R Jensen¹, A W Kuss⁴

Affiliations

¹ Human Molecular Genetics Group, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
² SensID GmbH, Rostock, Germany.
³ Human Molecular Genetics Group, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; Institute of Bioinformatics, University Medicine Greifswald, Greifswald, Germany.
⁴ Human Molecular Genetics Group, Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany. Electronic address: [email protected].

PMID: 38555715
DOI: 10.1016/j.scr.2024.103403

Abstract

We have created two isogenic iPSC lines from fibroblasts of a healthy male donor of European ancestry. The cell lines express common pluripotency markers, are free of chromosomal aberrations and are able to differentiate into cells of all three germ layers. These iPSC are now a resource for genome editing with the aim of creating models of genetic disorders without having to depend on patient cells.

MeSH terms

Cell Differentiation
Cell Line
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Male
White People