Exposure of small ruminants and humans to Coxiella burnetii in the semi-arid region of Northeastern Brazil

Braz J Microbiol. 2024 Jun;55(2):1931-1939. doi: 10.1007/s42770-024-01317-x. Epub 2024 Apr 4.

Abstract

Q fever is a zoonotic disease caused by the obligate intracellular pathogen Coxiella burnetii, for which domestic ruminants are the primary source of infection in humans. Herein, we investigated the presence of C. burnetii in humans, sheep, and goats in the semi-arid region of northeastern Brazil. The presence of anti-C. burnetii antibodies was surveyed using indirect immunofluorescence assay, and detection of C. burnetii DNA was performed by polymerase chain reaction (PCR). Anti-C. burnetii antibodies were detected in 60% of farms, 4.8% of goats, 1.5% of sheep, and 4.5% of human samples. PCR was positive in 18.9% of blood samples, 7.7% of milk samples, and 7.7% of vaginal mucus samples. A DNA sequence of a C. burnetii DNA sample extracted from the goat vaginal mucus showed 99.2-99.4% nucleotide identity with other strains previously reported in Brazil. These results indicate that C. burnetii is present in the surveyed area, where it poses a risk to both public and animal health. These findings indicate an urgent need for educative actions to protect population, as well as better training of veterinarians to detect and report Q fever.

Keywords: Epidemiology; Goat; Human; Q fever; Sheep; Zoonosis.

MeSH terms

  • Animals
  • Antibodies, Bacterial* / blood
  • Brazil / epidemiology
  • Coxiella burnetii* / genetics
  • Coxiella burnetii* / immunology
  • Coxiella burnetii* / isolation & purification
  • DNA, Bacterial / genetics
  • Female
  • Goat Diseases* / epidemiology
  • Goat Diseases* / microbiology
  • Goats* / microbiology
  • Humans
  • Q Fever* / epidemiology
  • Q Fever* / microbiology
  • Q Fever* / veterinary
  • Sheep
  • Sheep Diseases* / epidemiology
  • Sheep Diseases* / microbiology
  • Zoonoses / microbiology

Substances

  • Antibodies, Bacterial
  • DNA, Bacterial