Targeted G-to-T base editing for generation of novel herbicide-resistance gene alleles in rice

Yifu Tian; Xinbo Li; Jiyong Xie; Zai Zheng; Rundong Shen; Xuesong Cao; Mugui Wang; Chao Dong; Jian-Kang Zhu

doi:10.1111/jipb.13657

Targeted G-to-T base editing for generation of novel herbicide-resistance gene alleles in rice

J Integr Plant Biol. 2024 Jun;66(6):1048-1051. doi: 10.1111/jipb.13657. Epub 2024 Apr 5.

Authors

Yifu Tian^{1

2}, Xinbo Li^{1

2}, Jiyong Xie^{3

4}, Zai Zheng^{1

2}, Rundong Shen^{1

2}, Xuesong Cao⁵, Mugui Wang¹, Chao Dong^{1

2}, Jian-Kang Zhu^{1

5}

Affiliations

¹ Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572024, China.
² Hainan Seed Industry Laboratory, Sanya, 572024, China.
³ Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, The Chinese Academy of Sciences, Shanghai, 201602, China.
⁴ University of Chinese Academy of Sciences, Beijing, 100049, China.
⁵ Institute of Advanced Biotechnology, and School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China.

PMID: 38578176
DOI: 10.1111/jipb.13657

Abstract

A newly developed rice guanine base editor (OsGTBE) achieves targeted and efficient G-to-T editing (C-to-A in the opposite strand) in rice. Using OsGTBE to edit endogenous herbicide-resistant loci generated several novel alleles conferring herbicide resistance, highlighting its utility in creating valuable germplasm and enhancing genetic diversity..

MeSH terms

Alleles*
Base Sequence
Gene Editing* / methods
Genes, Plant
Herbicide Resistance* / genetics
Herbicides / pharmacology
Oryza* / genetics

Substances

Herbicides

Abstract

MeSH terms

Substances

Grants and funding