Rotary FoF1-ATP Synthase-Driven Flasklike Pentosan Colloidal Motors with ATP Synthesis and Storage

Yue Li; Jun Liu; Yingjie Wu; Qiang He

doi:10.1021/jacs.4c00334

Rotary F_oF₁-ATP Synthase-Driven Flasklike Pentosan Colloidal Motors with ATP Synthesis and Storage

J Am Chem Soc. 2024 Apr 10. doi: 10.1021/jacs.4c00334. Online ahead of print.

Authors

Yue Li¹, Jun Liu^{1

2}, Yingjie Wu¹, Qiang He¹

Affiliations

¹ School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China.
² Wenzhou Institute, University of Chinese Academy of Sciences, 1 Jinlian Street, Wenzhou 325000, China.

PMID: 38598314
DOI: 10.1021/jacs.4c00334

Abstract

We report the hierarchical assembly of a chloroplast-derived rotary F_oF₁-ATPase motor-propelled flasklike pentosan colloidal motor (FPCM) with the ability of the synthesis, storage, and triggered release of biological energy currency ATP. These streamlined and submicrometer-sized hollow flasklike pentosan colloidal motors are prepared by combining a soft-template-based hydrothermal polymerization with a vacuum infusion of chloroplast-derived proteoliposomes containing rotary F_oF₁-ATPase motors. The generation of proton motive force across the proteoliposomes by injecting an acidic buffer solution promotes the rotation of F_oF₁-ATPase motors to drive the self-propelled motion of FPCMs, accompanying the inner ATP synthesis and storage. These rotary F_oF₁-ATPase motor-powered FPCMs exhibit a chemotactic behavior by migrating from their neck opening to their round bottom along a proton gradient of the external environment (negative chemotaxis). Such rotary biomolecular motor-driven flasklike pentosan colloidal motors with ATP synthesis and on-demand release make them promising candidates for engineering novel intelligent nanocarriers to actively regulate cellular metabolism.