Directed and acyclic synaptic connectivity in the human layer 2-3 cortical microcircuit

Science. 2024 Apr 19;384(6693):338-343. doi: 10.1126/science.adg8828. Epub 2024 Apr 18.

Abstract

The computational capabilities of neuronal networks are fundamentally constrained by their specific connectivity. Previous studies of cortical connectivity have mostly been carried out in rodents; whether the principles established therein also apply to the evolutionarily expanded human cortex is unclear. We studied network properties within the human temporal cortex using samples obtained from brain surgery. We analyzed multineuron patch-clamp recordings in layer 2-3 pyramidal neurons and identified substantial differences compared with rodents. Reciprocity showed random distribution, synaptic strength was independent from connection probability, and connectivity of the supragranular temporal cortex followed a directed and mostly acyclic graph topology. Application of these principles in neuronal models increased dimensionality of network dynamics, suggesting a critical role for cortical computation.

MeSH terms

  • Animals
  • Humans
  • Nerve Net* / physiology
  • Nerve Net* / ultrastructure
  • Patch-Clamp Techniques
  • Pyramidal Cells* / physiology
  • Pyramidal Cells* / ultrastructure
  • Rodentia
  • Synapses* / physiology
  • Synapses* / ultrastructure
  • Temporal Lobe* / physiology