Extraction of Soluble Proteins from Leaves

Elizabete Carmo-Silva; Rhiannon Page; Catherine J Marsden; Armida Gjindali; Douglas J Orr

doi:10.1007/978-1-0716-3790-6_20

Extraction of Soluble Proteins from Leaves

Methods Mol Biol. 2024:2790:391-404. doi: 10.1007/978-1-0716-3790-6_20.

Authors

Elizabete Carmo-Silva¹, Rhiannon Page², Catherine J Marsden², Armida Gjindali², Douglas J Orr²

Affiliations

¹ Lancaster Environment Centre, Lancaster University, Lancaster, UK. [email protected].
² Lancaster Environment Centre, Lancaster University, Lancaster, UK.

PMID: 38649582
DOI: 10.1007/978-1-0716-3790-6_20

Abstract

Protein biochemistry can provide valuable answers to better understand plant performance and responses to the surrounding environment. In this chapter, we describe the process of extracting proteins from plant leaf samples. We highlight the key aspects to take into consideration to preserve protein integrity, from sample collection to extraction and preparation or storage for subsequent analysis of protein abundance and/or enzymatic activities.

Keywords: Calvin-Benson-Bassham cycle; Chloroplast proteins; Enzymatic activity; Photosynthetic metabolism; Protein biochemistry; Protein integrity; Protein quantification; Rubisco; Rubisco activase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Plant Leaves* / chemistry
Plant Proteins* / isolation & purification
Solubility

Substances

Plant Proteins