Exploring the Impact of Cigarette Smoke Extracts on Vitamin B12: Insights into the Transformation of Methylcobalamin and Hydroxycobalamin to Cyanocobalamin through In Vitro Evaluation

Mazhar Salim Al Zoubi; Mus'aab A Al-Oun; Fatima Yacoub Abusahyoun; Manal Issam Abualarja; Asmaa Al Smadi; Bahaa Al-Trad; Sura A Awadin; Khalid Al-Batayneh; Mai Elaarag; Raed M Al-Zoubi

doi:10.1155/2024/8827402

Exploring the Impact of Cigarette Smoke Extracts on Vitamin B₁₂: Insights into the Transformation of Methylcobalamin and Hydroxycobalamin to Cyanocobalamin through In Vitro Evaluation

Biochem Res Int. 2024 Apr 18:2024:8827402. doi: 10.1155/2024/8827402. eCollection 2024.

Affiliations

¹ Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid 211-63, Jordan.
² Department of Biological Sciences, Faculty of Sciences, Yarmouk University, Irbid 211-63, Jordan.
³ Surgical Research Section, Department of Surgery, Hamad Medical Corporation, Doha, Qatar.
⁴ Department of Chemistry, Jordan University of Science and Technology, Irbid 22110, Jordan.
⁵ Department of Biomedical Sciences, College of Health Sciences, Qatar University, Doha 2713, Qatar.

Abstract

Vitamin B₁₂ (cobalamin) is a water-soluble molecule required for the proper functioning of metabolism, blood and DNA synthesis, and neurological development. Vitamin B₁₂ exists in several forms: methylcobalamin (MeCbl), adenosylcobalamin (AdoCbl), hydroxycobalamin (OHCbl), and cyanocobalamin (CNCbl). This study aimed to evaluate the effect of cigarette smoke on the chemical structure of methylcobalamin and hydroxycobalamin forms of vitamin B₁₂. MeCbl and OHCbl were markedly affected by exposure to cigarette smoke. The resemblance of the Rt between MeCbl and OHCbl and CNCbl indicates that exposure to cigarette smoke extracts chemically alters MeCbl and OHCbl to CNCbl, warranting in vivo research investigations.