A Rapid, Equipment-Free DNA Isolation Method for DNA Barcoding

Methods Mol Biol. 2024:2744:517-523. doi: 10.1007/978-1-0716-3581-0_31.

Abstract

This rapid, equipment-free DNA isolation procedure using chromatography paper is a simple method that can be performed in less than 30 min and requires no wet lab experience. With minimal expense, it offers an affordable alternative for anyone wanting to explore biodiversity. It also provides an excellent option for use in classrooms or other activities that are time limited. The method works best for plants or lichens, producing stable DNA on Whatman® chromatography paper at room temperature, which can be eluted as needed.

Keywords: Biology; CURE; Chromatography paper; Community science; Course-based undergraduate research; DNA barcoding; DNA extraction; Education; High school research; Lichen-ITS; Plant; Rapid; Remote learning; rbcL.

MeSH terms

  • Chromatography / methods
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Barcoding, Taxonomic* / methods
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Lichens / genetics
  • Plants / genetics

Substances

  • DNA
  • DNA, Plant