Protocol for optical, aqueous-based clearing of murine tissues using EZ Clear

Affiliations

• ¹ Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
• ² Department of Human and Molecular Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
• ³ Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, USA.
• ⁴ Department of Integrative Physiology, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Advanced Technology Cores, Baylor College of Medicine, Houston, TX 77030, USA; Department of Education, Innovation and Technology, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: [email protected].
• ⁵ Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; Department of Neuroscience, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; Brain, Immunology and Glia (BIG) Center, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; UVA Comprehensive Cancer Center, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22903, USA. Electronic address: [email protected].