Isolation of model soluble immune complexes in the fluid phase by monoclonal anti-IgG-ferritin antibodies

Scand J Immunol. 1985 Jan;21(1):1-9. doi: 10.1111/j.1365-3083.1985.tb01396.x.

Abstract

Ferritin conjugates of monoclonal IgG anti-human gamma chain (anti-IgG-F) were reacted with soluble heat aggregates of IgG (A-IgG) and with soluble DNA-anti-DNA complexes to increase the S rate of the model soluble immune complexes (ICx) and thus facilitate isolation of ICx in the fluid phase and provide an immunochemical marker for subsequent ultrastructural analysis. A-IgG appeared as globular or curvilinear structures with individual IgG molecules arranged in a random fashion. The technique appears promising for characterization of other soluble ICx.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Antinuclear / immunology
  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Complex / isolation & purification*
  • Antigen-Antibody Reactions
  • DNA / immunology
  • Ferritins / immunology*
  • Hot Temperature
  • Humans
  • Immunoglobulin G / immunology*
  • Iodine Radioisotopes
  • Microscopy, Electron
  • Solubility

Substances

  • Antibodies, Antinuclear
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Immunoglobulin G
  • Iodine Radioisotopes
  • DNA
  • Ferritins