Measurement of HIV Rev-Rev Response Element Functional Activity

Patrick E H Jackson; Godfrey Dzhivhuho; Jing Huang; Marie-Louise Hammarskjold; David Rekosh

doi:10.1007/978-1-0716-3862-0_13

Measurement of HIV Rev-Rev Response Element Functional Activity

Methods Mol Biol. 2024:2807:175-194. doi: 10.1007/978-1-0716-3862-0_13.

Authors

Patrick E H Jackson^{1

2}, Godfrey Dzhivhuho^{3

4}, Jing Huang^{3

4}, Marie-Louise Hammarskjold^{3

4}, David Rekosh^{3

4}

Affiliations

¹ Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville, VA, USA. [email protected].
² Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA. [email protected].
³ Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville, VA, USA.
⁴ Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.

PMID: 38743229
DOI: 10.1007/978-1-0716-3862-0_13

Abstract

Retroviruses must overcome cellular restrictions to the nucleocytoplasmic export of viral mRNAs that retain introns in order to complete their replication cycle. HIV accomplishes this using a system comprised of a trans-acting viral protein, Rev, and a cis-acting RNA secondary structure in the viral genome, the Rev-Response Element (RRE). HIV primary isolates differ with respect to the sequence and functional activity of the Rev-RRE system. Here, we describe a high throughput assay system for analyzing Rev-RRE functional activity using packageable viral vectors.

Keywords: HIV; LentivirusLentiviruses; RNA traffickingRNA trafficking; Rev; Rev response elementRev response element.

MeSH terms

Gene Expression Regulation, Viral
Genetic Vectors / genetics
HIV-1 / genetics
HIV-1 / physiology
Humans
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Viral* / genetics
Response Elements* / genetics
Virus Replication / genetics
rev Gene Products, Human Immunodeficiency Virus* / genetics
rev Gene Products, Human Immunodeficiency Virus* / metabolism