Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows

Iago Pereiro; Anna Fomitcheva-Khartchenko; Govind V Kaigala

doi:10.1007/978-1-0716-3850-7_6

Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows

Methods Mol Biol. 2024:2804:103-115. doi: 10.1007/978-1-0716-3850-7_6.

Authors

Iago Pereiro¹, Anna Fomitcheva-Khartchenko², Govind V Kaigala^{3

4}

Affiliations

¹ IBM Research Europe, Rüschlikon, Switzerland. [email protected].
² IBM Research Europe, Rüschlikon, Switzerland.
³ IBM Research Europe, Rüschlikon, Switzerland. [email protected].
⁴ School of Biomedical Engineering, Vancouver Prostate Center, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada. [email protected].

PMID: 38753143
DOI: 10.1007/978-1-0716-3850-7_6

Abstract

In this chapter, we present the design and fabrication of a device and implementation of a protocol to realize increased efficiency of immunoassays within microtiter plates. The device, WellProbe, is a 3D-structured probe that can be used to deliver precise flows at the bottom of standard well plates to establish concentric areas of shear stress intensities using hydrodynamically confined flows. The protocols involve both operation and data analysis.

Keywords: Advection; Mass transport; Microtiter plate; Multiplexing; Open-space microfluidics; Surface assays.

MeSH terms

Equipment Design*
Humans
Hydrodynamics
Immunoassay / instrumentation
Immunoassay / methods
Microfluidic Analytical Techniques / instrumentation
Microfluidic Analytical Techniques / methods