1-Methyl-4-phenyl-pyridinium-induced inhibition of nicotinamide adenosine dinucleotide cytochrome c reductase

Neurosci Lett. 1985 Nov 20;62(1):7-11. doi: 10.1016/0304-3940(85)90276-9.

Abstract

The effect of 1-methyl-4-phenyl-pyridinium (MPP+), the main toxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), a parkinsonism-causing neurotoxin, upon the activity of nicotinamide adenine dinucleotide (NADH) cytochrome c reductase (EC 1.6.99.3) and upon that of glutathione reductase (EC 1.6.4.2) was monitored spectrophotometrically. For the cytochrome c reductase, the increase in absorbance of reduced cytochrome c was measured at 550 nm; for evaluating glutathione reductase, the absorbance of nicotinamide adenine dinucleotide phosphate (NADPH) was followed at 340 nm. MPP+ but not MPTP reversibly inhibited NADH cytochrome c reductase, but not glutathione reductase. This may be a direct mechanism of cell toxicity by this neurotoxin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • 1-Methyl-4-phenylpyridinium
  • Animals
  • Cytochrome Reductases / antagonists & inhibitors*
  • Glutathione Reductase / metabolism
  • NADH Dehydrogenase / antagonists & inhibitors*
  • Parkinson Disease, Secondary / chemically induced
  • Pyridines / metabolism
  • Pyridines / pharmacology
  • Pyridinium Compounds / metabolism
  • Pyridinium Compounds / pharmacology*
  • Spectrophotometry

Substances

  • Pyridines
  • Pyridinium Compounds
  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • Cytochrome Reductases
  • NADH Dehydrogenase
  • Glutathione Reductase
  • 1-Methyl-4-phenylpyridinium