Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology

Yanying Wang; Yixuan Liu; Chengqun Yu; Shizhan Chen; Yankun Li; Lina Wei; Junxi Wu; Jianping Yang

doi:10.3390/genes15050572

Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology

Genes (Basel). 2024 Apr 29;15(5):572. doi: 10.3390/genes15050572.

Authors

Yanying Wang^{1

2

3}, Yixuan Liu¹, Chengqun Yu², Shizhan Chen³, Yankun Li³, Lina Wei³, Junxi Wu^{1

2}, Jianping Yang³

Affiliations

¹ Key Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa 850000, China.
² Lhasa Plateau Ecosystem Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.
³ Center for Crop Genome Engineering, State Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.

Abstract

Wintergrazer-70 and Ganyin No1 are high-yield forage varieties suitable for cultivation in high-altitude areas of Tibet (4300 m above sea level). Ganyin No1 was developed from Wintergrazer-70, with the latter serving as its parent variety. Ganyin No1 was identified as a spring variety, and subsequent RNA sequencing was conducted. RNA sequencing analysis identified 4 differentially expressed genes related to vernalization and 28 genes related to photoperiod regulation. The Sc7296g5-i1G3 gene is related to the flowering inhibition of rye, which may be related to the phenotypic difference in the Ganyin No1 variety in winter and spring. This finding provides valuable insights for future research on Ganyin No1, especially in addressing feed shortages in Tibet during winter and spring.

Keywords: RNA-seq; Secale cereal L.; Tibet; winter–spring characteristic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Altitude*
Gene Expression Regulation, Plant
Mutation
Plant Proteins / genetics
Seasons*
Secale* / genetics
Sequence Analysis, RNA / methods
Tibet

Substances

Plant Proteins

Grants and funding

The National Key Research and Development Program Project (funder: Junxi Wu; funding number: 2021YFD1000303); Major Special Projects of Science and Technology Plan in Tibet Autonomous Region (funder: Junxi Wu; funding number: XZ202101ZD003N); Lhasa Regional Science and Technology Collaborative Innovation Project in 2022 (funder: Jianping Yang; funding number: QYXTZX-LS2022-01); and High-Level Talent Cultivation Program for Postgraduates of Tibet University (funder: Yanying Wang; funding number: 2021-GSP-S045) supported this research.