Diet/photoperiod mediated changes in cerebellar clock genes causes locomotor shifts and imperative changes in BDNF-TrkB pathway

Neurosci Lett. 2024 Jul 13:835:137843. doi: 10.1016/j.neulet.2024.137843. Epub 2024 May 29.

Abstract

Neuropsychological studies report anxiety and depression like symptoms in patients suffering from lifestyle disorder but its impact on locomotor function lacks clarity. Our study investigates locomotor deficits resulting due to perturbations in cerebellum of high fat diet (HFD), chronodisruption (CD) or a combination (HCD) model of lifestyle disorder. Significant downregulation in levels of cerebellar clock genes (Bmal-1, Clock, Per 1 and Per 2) and Bdnf-Trkb pathway genes (Bdnf, TrkB and Syn1 levels) were recorded. Further, locomotor deficits were observed in all the three experimental groups as evidenced by actimeter test, pole test and wire hanging test. Nuclear pyknosis of Purkinje cells, their derangement and inflammation were the hallmark of cerebellar tissue of all the three experimental groups. Taken together, this study generates important links between cerebellar clock oscillations, locomotor function and Bdnf-TrkB signaling.

Keywords: Bdnf-Trkb pathway; Chronodisruption; High fat diet; Lifestyle Disorder; Locomotor deficits; Neurobehavior.

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor* / genetics
  • Brain-Derived Neurotrophic Factor* / metabolism
  • Cerebellum* / metabolism
  • Diet, High-Fat / adverse effects
  • Locomotion / physiology
  • Male
  • Purkinje Cells / metabolism
  • Receptor, trkB* / genetics
  • Receptor, trkB* / metabolism
  • Signal Transduction*

Substances

  • Brain-Derived Neurotrophic Factor
  • Receptor, trkB