Cutting-edge collagen biocomposite reinforced with 2D nano-talc for bone tissue engineering

Nanomedicine. 2024 Aug:60:102756. doi: 10.1016/j.nano.2024.102756. Epub 2024 Jun 6.

Abstract

The advancement of nanobiocomposites reinforced with 2D nano-materials plays a pivotal role in enhancing bone tissue engineering. In this study, we introduce a nanobiocomposite that reinforces bovine collagen with 2D nano-talc, a recently exfoliated nano-mineral. These nanobiocomposites were prepared by blending collagen with varying concentrations of 2D nano-talc, encompassing mono- and few-layers talc from soapstone nanomaterial. Extensive characterization techniques including AFM, XPS, nano-FTIR, s-SNOM nanoimaging, Force Spectroscopy, and PeakForce QNM® were employed. The incorporation of 2D nano-talc significantly enhanced the mechanical properties of the nanobiocomposites, resulting in increased stiffness compared to pristine collagen. In vitro studies supported the growth and proliferation of osteoblasts onto 2D nano-talc-reinforced nanobiocomposites, as well as showed the highest mineralization potential. These findings highlight the substantial potential of the developed nanobiocomposite as a scaffold material for bone tissue engineering applications.

Keywords: 2D nano-talc; Biocompatibility; Collagen; Nanobiomaterial; Nanomechanical analysis.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Bone and Bones*
  • Cattle
  • Cell Proliferation / drug effects
  • Collagen* / chemistry
  • Humans
  • Nanocomposites* / chemistry
  • Osteoblasts* / cytology
  • Osteoblasts* / drug effects
  • Tissue Engineering* / methods
  • Tissue Scaffolds / chemistry

Substances

  • Collagen
  • Biocompatible Materials