Generation of a human iPSC cell line (MUSIi017-A) from a donor with O negative blood type

Stem Cell Res. 2024 Aug:78:103466. doi: 10.1016/j.scr.2024.103466. Epub 2024 Jun 6.

Abstract

The Rh-negative type O blood group (O Rh-) is considered a universal donor for emergency blood transfusions. Due to the constant shortage of this rare blood group, the production of blood cells from iPSCs derived from the O Rh- donor could potentially serve as a limitless blood source for transfusions. In this report, we establish a MUSIi017-A iPSC line from peripheral blood mononuclear cells of a healthy donor with the O Rh- blood group. The established iPSC line exhibited a normal karyotype, showed identical STR compared to donor peripheral blood mononuclear cells, and could differentiate to all three germ layers.

Keywords: blood transfusion; iPSCs; universal donor.

MeSH terms

  • ABO Blood-Group System
  • Blood Donors
  • Cell Differentiation
  • Cell Line
  • Humans
  • Induced Pluripotent Stem Cells* / cytology
  • Induced Pluripotent Stem Cells* / metabolism
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / metabolism

Substances

  • ABO Blood-Group System