Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on 15N-Labelled Ammonium Ions

Carl Öster; Sascha Lange; Kitty Hendriks; Adam Lange

doi:10.1007/978-1-0716-3818-7_2

Detecting Bound Ions in Ion Channels by Solid-State NMR Experiments on ¹⁵N-Labelled Ammonium Ions

Methods Mol Biol. 2024:2796:23-34. doi: 10.1007/978-1-0716-3818-7_2.

Authors

Carl Öster¹, Sascha Lange², Kitty Hendriks², Adam Lange^{3

4}

Affiliations

¹ Research Unit Molecular Biophysics, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. [email protected].
² Research Unit Molecular Biophysics, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.
³ Research Unit Molecular Biophysics, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany. [email protected].
⁴ Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany. [email protected].

PMID: 38856893
DOI: 10.1007/978-1-0716-3818-7_2

Abstract

Solid-state NMR allows for the study of membrane proteins under physiological conditions. Here we describe a method for detection of bound ions in the selectivity filter of ion channels using solid-state NMR. This method employs standard ¹H-detected solid-state NMR setup and experiment types, which is enabled by using ¹⁵N-labelled ammonium ions to mimic potassium ions.

Keywords: Ammonium; Ion binding; Ion channels; Membrane proteins; Solid-state NMR.

MeSH terms

Ammonium Compounds* / analysis
Ammonium Compounds* / chemistry
Ion Channels* / chemistry
Ion Channels* / metabolism
Ions / chemistry
Magnetic Resonance Spectroscopy / methods
Nitrogen Isotopes* / analysis
Nuclear Magnetic Resonance, Biomolecular / methods

Substances

Nitrogen Isotopes
Ammonium Compounds
Ion Channels
Ions
Nitrogen-15