Two Undescribed Coumarins from Notopterygium Incisum with Anti-Inflammatory Activity

Chem Biodivers. 2024 Aug;21(8):e202401093. doi: 10.1002/cbdv.202401093. Epub 2024 Jul 30.

Abstract

Two previously undescribed coumarins (1-2) were isolated from the root of Notopterygium incisum. The structures of new findings were elucidated by analyses of spectral evidences in HRESIMS, NMR, as well as ICD. The absolute configurations were further confirmed by chemical calculations. 1-2 exhibits obviously anti-inflammatory activity by inhibiting the expression of inflammatory mediators (COX-2, iNOS), as well as reducing the release of NO and the accumulation of ROS in cells. Western blotting analysis revealed that 2 could inhibit the PI3K/AKT pathway by reducing the expression of p-PI3K and p-AKT.

Keywords: Notopterygium incisum; PI3K/AKT pathway; anti-inflammatory activity; coumarin.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents, Non-Steroidal / chemistry
  • Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Apiaceae* / chemistry
  • Coumarins* / chemistry
  • Coumarins* / isolation & purification
  • Coumarins* / pharmacology
  • Cyclooxygenase 2 / metabolism
  • Dose-Response Relationship, Drug
  • Lipopolysaccharides / antagonists & inhibitors
  • Lipopolysaccharides / pharmacology
  • Mice
  • Molecular Structure
  • Nitric Oxide Synthase Type II* / antagonists & inhibitors
  • Nitric Oxide Synthase Type II* / metabolism
  • Nitric Oxide* / antagonists & inhibitors
  • Nitric Oxide* / biosynthesis
  • Nitric Oxide* / metabolism
  • Nitriles / chemistry
  • Phosphatidylinositol 3-Kinases / metabolism
  • Plant Roots / chemistry
  • Proto-Oncogene Proteins c-akt / antagonists & inhibitors
  • Proto-Oncogene Proteins c-akt / metabolism
  • RAW 264.7 Cells
  • Reactive Oxygen Species / antagonists & inhibitors
  • Reactive Oxygen Species / metabolism
  • Structure-Activity Relationship

Substances

  • Anti-Inflammatory Agents
  • Anti-Inflammatory Agents, Non-Steroidal
  • Coumarins
  • Cyclooxygenase 2
  • Lipopolysaccharides
  • Nitric Oxide
  • Nitric Oxide Synthase Type II
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • Reactive Oxygen Species
  • Nitriles