An Injectable Hydrogel with Ultrahigh Burst Pressure and Innate Antibacterial Activity for Emergency Hemostasis and Wound Repair

Adv Mater. 2024 Aug;36(33):e2404811. doi: 10.1002/adma.202404811. Epub 2024 Jun 22.

Abstract

Uncontrolled bleeding and wound infections following severe trauma pose significant challenges for existing tissue adhesives, primarily due to their weak wet adhesion, slow adhesion formation, cytotoxicity concerns, and lack of antibacterial properties. Herein, an injectable hydrogel (denoted as ES gel) with rapid, robust adhesive sealing and inherent antibacterial activity based on ε-polylysine and a poly(ethylene glycol) derivative is developed. The engineered hydrogel exhibits rapid gelation behavior, high mechanical strength, strong adhesion to various tissues, and can sustain an ultrahigh burst pressure of 450 mmHg. It also presents excellent biocompatibility, biodegradability, antibacterial properties, and on-demand removability. Significantly improved hemostatic efficacy of ES gel compared to fibrin glue is demonstrated using various injury models in rats and rabbits. Remarkably, the adhesive hydrogel can effectively halt lethal non-compressible hemorrhages in visceral organs (liver, spleen, and heart) and femoral artery injury models in fully anticoagulated pigs. Furthermore, the hydrogel outperforms commercial products in sutureless wound closure and repair in the rat liver defect, skin incision, and infected full-thickness skin wound models. Overall, this study highlights the promising clinical applications of ES gel for managing uncontrolled hemorrhage, sutureless wound closure, and infected wound repair.

Keywords: hemostatic hydrogels; inherent antibacterial activity; injectable adhesives; tissue repair; wound healing.

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Anti-Bacterial Agents* / therapeutic use
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Hemorrhage / drug therapy
  • Hemostasis* / drug effects
  • Hydrogels* / chemistry
  • Hydrogels* / pharmacology
  • Injections
  • Polyethylene Glycols / chemistry
  • Polylysine / chemistry
  • Polylysine / pharmacology
  • Pressure
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • Swine
  • Tissue Adhesives / chemistry
  • Tissue Adhesives / pharmacology
  • Tissue Adhesives / therapeutic use
  • Wound Healing* / drug effects

Substances

  • Hydrogels
  • Anti-Bacterial Agents
  • Polylysine
  • Polyethylene Glycols
  • Biocompatible Materials
  • Tissue Adhesives