Mechanisms of anammox granular sludge reactor effluent as biostimulant: Shaping microenvironment for anammox metabolism

Mengjiao Gao; Bing Guo; Xin Zou; Hengbo Guo; Yiduo Yao; Youpeng Chen; Jinsong Guo; Yang Liu

doi:10.1016/j.biortech.2024.130962

Mechanisms of anammox granular sludge reactor effluent as biostimulant: Shaping microenvironment for anammox metabolism

Bioresour Technol. 2024 Aug:406:130962. doi: 10.1016/j.biortech.2024.130962. Epub 2024 Jun 12.

Authors

Mengjiao Gao¹, Bing Guo², Xin Zou³, Hengbo Guo⁴, Yiduo Yao⁴, Youpeng Chen⁵, Jinsong Guo⁵, Yang Liu⁶

Affiliations

¹ College of Environment and Ecology, Chongqing University, Chongqing 400044, China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.
² Centre for Environmental Health and Engineering (CEHE), School of Sustainability, Civil and Environmental Engineering, University of Surrey, Guildford GU2 7XH, UK.
³ Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada; School of Civil & Environmental Engineering, Queensland University of Technology, Brisbane QLD 4001, Queensland, Australia.
⁴ Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.
⁵ College of Environment and Ecology, Chongqing University, Chongqing 400044, China.
⁶ Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada; School of Civil & Environmental Engineering, Queensland University of Technology, Brisbane QLD 4001, Queensland, Australia. Electronic address: [email protected].

PMID: 38876278
DOI: 10.1016/j.biortech.2024.130962

Abstract

Effluent from anammox granular sludge (AnGS) bioreactor contains microbes and microbial products. This study explored mechanisms of utilizing AnGS-effluent as biostimulant for anammox process enhancement. Compared with no AnGS-effluent supplemented control reactor, 5.0 and 1.3 times higher ammonium nitrogen and total inorganic nitrogen removal rates, respectively were obtained with continuous AnGS-effluent supplementation after 98 days' operation. Anammox bacteria from Candidatus Brocadia accounted for 0.1 % (DNA level) and 1.3 %-1.5 % (RNA level) in control reactor, and 2.9 % (DNA level) and 54.5 %-55.4 % (RNA level) in the AnGS-effluent-fed reactor. Influent microbial immigration evaluation showed that bacterial immigration via AnGS-effluent supplementation was not the main contributor to active anammox community development. Amino acids biosynthesis, B-vitamins and coenzymes metabolism related pathways were facilitated by AnGS-effluent supplementation. AnGS-effluent supplementation aided anammox metabolic activity by shaping microenvironment and microbial interactions. This study provides insights into enhancing anammox bacterial metabolism with AnGS-effluent microbial products as biostimulant.

Keywords: Anammox activity; Microbial community; Microbial immigration; Microbial products.

MeSH terms

Amino Acids / biosynthesis
Anaerobic Ammonia Oxidation*
Bacteria / metabolism
Bioreactors*
Nitrogen / analysis
Nitrogen / metabolism
Waste Disposal, Fluid* / methods
Water Purification / methods

Substances

Amino Acids
Nitrogen