Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantification and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.
Keywords: CD8+ T-cells; Cytokine secretion assay; Flow cytometry; Rotavirus; SARS-CoV-2; T-cell epitopes; T-cell staining; Tetramers.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.