Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors

David L Hacker; Laurence Durrer; Soraya Quinche; Kelvin Lau; Florence Pojer

doi:10.1007/978-1-0716-3878-1_6

Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors

Methods Mol Biol. 2024:2810:85-98. doi: 10.1007/978-1-0716-3878-1_6.

Authors

David L Hacker¹, Laurence Durrer¹, Soraya Quinche¹, Kelvin Lau¹, Florence Pojer²

Affiliations

¹ Protein Production and Structure Core Facility (PTPSP), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
² Protein Production and Structure Core Facility (PTPSP), Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. [email protected].

PMID: 38926274
DOI: 10.1007/978-1-0716-3878-1_6

Abstract

Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most important mammalian hosts for the production of recombinant proteins. In this chapter, the suspension cultivation and transfection of these cells in small-scale, single-use orbitally shaken bioreactors, TubeSpin™ bioreactor 50 [orbitally shaken reactor 50 (OSR50)], and TubeSpin™ bioreactor 600 [orbitally shaken reactor 600 (OSR600)] are described. These are conical centrifuge tubes with nominal volumes of 50 mL and 600 mL, respectively, that have been redesigned with a ventilated cap for the cultivation of animal cells in suspension at working volumes up to 20 mL and 400 mL, respectively.

Keywords: ExpiCHO cells; HEK293E cells; Polyethyleneimine; Single-use bioreactors; Suspension cultivation; Transfection.

MeSH terms

Animals
Bioreactors*
CHO Cells
Cell Culture Techniques / methods
Cricetulus*
HEK293 Cells
Humans
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Transfection* / methods

Substances

Recombinant Proteins