Synthesis of Structural ADP-Ribose Analogues as Inhibitors for SARS-CoV-2 Macrodomain 1

Org Lett. 2024 Jul 12;26(27):5700-5704. doi: 10.1021/acs.orglett.4c01792. Epub 2024 Jun 27.

Abstract

Protein adenosine diphosphate (ADP)-ribosylation is crucial for a proper immune response. Accordingly, viruses have evolved ADP-ribosyl hydrolases to remove these modifications, a prominent example being the SARS-CoV-2 NSP3 macrodomain, "Mac1". Consequently, inhibitors are developed by testing large libraries of small molecule candidates, with considerable success. However, a relatively underexplored angle in design pertains to the synthesis of structural substrate mimics. Here, we present the synthesis and biophysical activity of novel adenosine diphosphate ribose (ADPr) analogues as SARS-CoV-2 NSP3 Mac1 inhibitors.

MeSH terms

  • Adenosine Diphosphate Ribose* / chemistry
  • Adenosine Diphosphate Ribose* / metabolism
  • Antiviral Agents* / chemical synthesis
  • Antiviral Agents* / chemistry
  • Antiviral Agents* / pharmacology
  • COVID-19 Drug Treatment
  • Humans
  • Molecular Structure
  • Protein Domains
  • SARS-CoV-2* / drug effects

Substances

  • Adenosine Diphosphate Ribose
  • Antiviral Agents