Long-term storage stability of PGL-I coated ELISA plates for anti-Mycobacterium leprae IgM detection

J Microbiol Methods. 2024 Sep:224:106985. doi: 10.1016/j.mimet.2024.106985. Epub 2024 Jul 2.

Abstract

The assessment of ELISA plates coated with phenolic glycolipid-I/PGL-I revealed excellent stability during eight years of storage at room temperature, promoting consistent IgM antibody detection in multibacillary leprosy patients. These stable, standardized plates can significantly contribute to efficient leprosy serology research and support its widespread distribution and use in endemic countries.

Keywords: Diagnostics; IgM serology; Immunoassay stability; Immunobiotechnology; Room temperature storage; Synthetic antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial* / immunology
  • Antigens, Bacterial* / immunology
  • Enzyme-Linked Immunosorbent Assay* / methods
  • Glycolipids* / immunology
  • Humans
  • Immunoglobulin M* / blood
  • Leprosy / diagnosis
  • Leprosy / immunology
  • Leprosy / microbiology
  • Mycobacterium leprae* / immunology
  • Time Factors

Substances

  • Immunoglobulin M
  • Antibodies, Bacterial
  • phenolic glycolipid I, Mycobacterium leprae
  • Glycolipids
  • Antigens, Bacterial