Comparative Morphological and Molecular Genetic Characteristics of Cell and Tissue Strains of Experimental Rat Glioma 10-17-2 (Astrid-17)

A I Alekseeva; V V Kudelkina; A S Khalansky; A V Sentyabreva; E A Miroshnichenko; M V Gulyaev; K A Rakitina; A M Kosyreva

doi:10.1007/s10517-024-06150-8

Comparative Morphological and Molecular Genetic Characteristics of Cell and Tissue Strains of Experimental Rat Glioma 10-17-2 (Astrid-17)

Bull Exp Biol Med. 2024 May;177(1):169-175. doi: 10.1007/s10517-024-06150-8. Epub 2024 Jul 4.

Authors

A I Alekseeva¹, V V Kudelkina², A S Khalansky², A V Sentyabreva^{2

3}, E A Miroshnichenko^{2

3}, M V Gulyaev⁴, K A Rakitina³, A M Kosyreva^{2

3}

Affiliations

¹ Avtsyn Research Institute of Human Morphology, Petrovsky Russian Research Center of Surgery, Moscow, Russia. [email protected].
² Avtsyn Research Institute of Human Morphology, Petrovsky Russian Research Center of Surgery, Moscow, Russia.
³ Medical Institute, RUDN University, Moscow, Russia.
⁴ Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia.

PMID: 38960964
DOI: 10.1007/s10517-024-06150-8

Abstract

In order to obtain models of gliomas of varying degrees of malignancy, we performed morphological and molecular genetic study of a tissue strain of glioma 10-17-2 (Astrid-17) obtained by intracranial passaging of tumor fragments of chemically induced rat brain tumor, and a cell strain isolated from it. More or less pronounced changes in the expression levels of Mki67, Trp53, Vegfa, and Gfap genes in the tissue and cell strain of glioma 10-17-2 (Astrid-17) compared with intact brain tissue were shown. The tissue model of glioma 10-17-2 (Astrid-17) according to the studied characteristics shows features of grade 3-4 astrocytoma and the cellular model - grade 2-3 astrocytoma.

Keywords: Astrid-17; astrocytoma; astroglia; glioma; proliferation.

Publication types

Comparative Study

MeSH terms

Animals
Astrocytoma / genetics
Astrocytoma / metabolism
Astrocytoma / pathology
Brain / metabolism
Brain / pathology
Brain Neoplasms* / genetics
Brain Neoplasms* / metabolism
Brain Neoplasms* / pathology
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Glial Fibrillary Acidic Protein* / genetics
Glial Fibrillary Acidic Protein* / metabolism
Glioma* / genetics
Glioma* / metabolism
Glioma* / pathology
Ki-67 Antigen / genetics
Ki-67 Antigen / metabolism
Male
Rats
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism
Vascular Endothelial Growth Factor A* / genetics
Vascular Endothelial Growth Factor A* / metabolism

Substances

Glial Fibrillary Acidic Protein
Vascular Endothelial Growth Factor A
Tumor Suppressor Protein p53
Ki-67 Antigen
Gfap protein, rat
vascular endothelial growth factor A, rat