Development of a colloidal gold immunochromatographic strip for the simultaneous detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus

Jinzhu Zhou; Wei Wu; Dandan Wang; Wei Wang; Xinjian Chang; Yunchuan Li; Jizong Li; Baochao Fan; Junming Zhou; Rongli Guo; Xuejiao Zhu; Bin Li

doi:10.3389/fmicb.2024.1418959

Development of a colloidal gold immunochromatographic strip for the simultaneous detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus

Front Microbiol. 2024 Jun 19:15:1418959. doi: 10.3389/fmicb.2024.1418959. eCollection 2024.

Authors

Jinzhu Zhou^#^{1

2

3}, Wei Wu^#⁴, Dandan Wang^#^{1

2

3}, Wei Wang^{1

2

3}, Xinjian Chang^{1

2

3}, Yunchuan Li^{1

2

3}, Jizong Li^{1

2

3}, Baochao Fan^{1

2

3}, Junming Zhou^{1

2

3}, Rongli Guo^{1

2

3}, Xuejiao Zhu^{1

2

3}, Bin Li^{1

2

3}

Affiliations

¹ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
² Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, China.
³ Guotai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, China.
⁴ Fujian Agricultural and Forestry University, Fuzhou, China.

^# Contributed equally.

Abstract

In recent years, porcine diarrhea-associated viruses have caused significant economic losses globally. These viruses present similar clinical symptoms, such as watery diarrhea, dehydration, and vomiting. Co-infections with porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are common. For the rapid and on-site preliminary diagnosis on the pig farms, this study aimed to develop a colloidal gold immunochromatography assay (GICA) strip for the detection of PEDV and TGEV simultaneously. The GICA kit showed that there was no cross-reactivity with the other five common porcine viruses. With visual observation, the lower limits were approximately 104 TCID50/mL and 104 TCID50/mL for PEDV and TGEV, respectively. The GICA strip could be stored at 4°C or 25°C for 12 months without affecting its efficacy. To validate the GICA strip, 121 clinical samples were tested. The positive rates of PEDV and TGEV were 42.9 and 9.9%, respectively, and the co-infection rate of the two viruses was 5.8% based on the duplex GICA strip. Thus, the established GICA strip is a rapid, specific, and stable tool for on-site preliminary diagnosis of PEDV- and TGEV-associated diarrhea.

Keywords: RT-PCR; colloidal gold immunochromatographic strip assay; monoclonal antibodies; porcine epidemic diarrhea virus; transmissible gastroenteritis virus.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was supported by the National Key Research and Development Program (2023YFD1800502), the National Natural Science Foundation of China (32373030 and 32202787), the Natural Sciences Foundation of Jiangsu Province (BK20221432 and BK20210158), the China Postdoctoral Science Foundation (Grant No. 2022 M711398, 2022 M711398), the Jiangsu Agricultural Science and Technology Innovation Fund (CX (21) 3139), and the Innovation Foundation of Jiangsu Academy of Agricultural Sciences (ZX (21)1217). The funders had no role in the design of the study; in the collection, analysis, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.