Design, synthesis and structure-activity relationships of novel non-ketolides: 9-Oxime clarithromycin featured with seven-to thirteen-atom-length diamine linkers at 3-OH

Eur J Med Chem. 2024 Oct 5:276:116630. doi: 10.1016/j.ejmech.2024.116630. Epub 2024 Jun 25.

Abstract

We report here on the structure-activity relationships of hybrids combining 3-descladinosyl clarithromycin with quinolones linked by extended diamine connectors. Several hybrids, exemplified by 23Bc, 23Be, 23Bf, 26Be, and 30Bc, not only restored potency against inducibly resistant pathogens but also exhibited significantly enhanced activities against constitutively resistant strains of Staphylococcus pneumoniae and Staphylococcus pyogenes, which express high-level resistance independent of clarithromycin or erythromycin induction. Additionally, the novel hybrids showed susceptibility against Gram-negative Haemophilus influenzae. Notably, hybrid 23Be demonstrated dual modes of action by inhibiting both protein synthesis and DNA replication in vitro and in vivo. Given these promising characteristics, 23Be emerges as a potential candidate for the treatment of community-acquired bacterial pneumonia.

Keywords: Ciprofloxacin; Erythromycin; Macrolide; Quinolone; Resistant bacteria; Ribosome.

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemical synthesis
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Clarithromycin* / chemical synthesis
  • Clarithromycin* / chemistry
  • Clarithromycin* / pharmacology
  • Diamines / chemical synthesis
  • Diamines / chemistry
  • Diamines / pharmacology
  • Dose-Response Relationship, Drug
  • Drug Design*
  • Drug Resistance, Bacterial / drug effects
  • Haemophilus influenzae / drug effects
  • Humans
  • Microbial Sensitivity Tests*
  • Molecular Structure
  • Oximes / chemical synthesis
  • Oximes / chemistry
  • Oximes / pharmacology
  • Streptococcus pyogenes / drug effects
  • Structure-Activity Relationship

Substances

  • Clarithromycin
  • Anti-Bacterial Agents
  • Diamines
  • Oximes