Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae)

Wagner Campos Otoni; Jéssica Ribeiro Soares; Claudinei Silva Souza; Lázara Aline Simões Silva; Lana Laene Lima Dias; Kerly Jessenia Moncaleano Robledo; Daniela Lopes Paim-Pinto; Andréa Dias Koehler; Pedro Alexandre Sodrzeieski; Amanda Mendes Fernandes; Lorena Melo Vieira; Priscila Oliveira Silva; Emerson Campos Silveira; Elyabe Monteiro Matos; Ilio Fealho Carvalho; Elisson Romanel; Diego Silva Batista; Lyderson Facio Viccini; Fábio Gelape Faleiro; Diego Ismael Rocha; Fábio Tebaldi Silveira Nogueira; Maurecilne Lemes Carvalho Silva

doi:10.1007/978-1-0716-3954-2_15

Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae)

Methods Mol Biol. 2024:2827:207-222. doi: 10.1007/978-1-0716-3954-2_15.

Authors

Wagner Campos Otoni¹, Jéssica Ribeiro Soares^#², Claudinei Silva Souza^#², Lázara Aline Simões Silva^#², Lana Laene Lima Dias^#², Kerly Jessenia Moncaleano Robledo², Daniela Lopes Paim-Pinto³, Andréa Dias Koehler², Pedro Alexandre Sodrzeieski², Amanda Mendes Fernandes⁴, Lorena Melo Vieira², Priscila Oliveira Silva⁵, Emerson Campos Silveira², Elyabe Monteiro Matos⁶, Ilio Fealho Carvalho⁷, Elisson Romanel⁸, Diego Silva Batista⁹, Lyderson Facio Viccini⁶, Fábio Gelape Faleiro¹⁰, Diego Ismael Rocha¹¹, Fábio Tebaldi Silveira Nogueira¹², Maurecilne Lemes Carvalho Silva⁷

Affiliations

¹ Laboratório de Cultura de Tecidos-LCT/BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil. [email protected].
² Laboratório de Cultura de Tecidos-LCT/BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
³ Department of Biological Sciences, Center for Biotechnology and Life Sciences, The University of Rhode Island, Kingston, RI, USA.
⁴ Lethbridge College, Lethbridge, AB, Canada.
⁵ Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
⁶ Laboratório de Genética e Biotecnologia, ICB, Departamento de Biologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
⁷ Departamento de Biologia, Universidade do Estado de Mato Grosso, Tangará da Serra, MT, Brazil.
⁸ Laboratório de Genômica de Plantas e Bioenergia (PGEMBL), Escola de Engenharia de Lorena (EEL), Universidade de São Paulo (USP), Lorena, SP, Brazil.
⁹ Departamento de Agricultura, Universidade Federal da Paraíba, Campus Universitário III, S/N, Bananeiras, PB, Brazil.
¹⁰ Empresa Brasileira de Pesquisa Agropecuária-Embrapa Cerrados, Planaltina, DF, Brazil.
¹¹ Departamento de Agronomia, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
¹² Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Piracicaba, SP, Brazil.

^# Contributed equally.

PMID: 38985273
DOI: 10.1007/978-1-0716-3954-2_15

Abstract

In this chapter, we report advances in tissue culture applied to Passiflora. We present reproducible protocols for somatic embryogenesis, endosperm-derived triploid production, and genetic transformation for such species knowledge generated by our research team and collaborators in the last 20 years. Our research group has pioneered the work on passion fruit somatic embryogenesis, and we directed efforts to characterize several aspects of this morphogenic pathway. Furthermore, we expanded the possibilities of understanding the molecular mechanism related to developmental phase transitions of Passiflora edulis Sims. and P. cincinnata Mast., and a transformation protocol is presented for the overexpression of microRNA156.

Keywords: Developmental phase transitions; Endosperm culture; Genetic transformation; In vitro morphogenesis; MicroRNAs; Passiflora; Somatic embryogenesis; Triploids.

MeSH terms

Endosperm / genetics
Endosperm / growth & development
Gene Expression Regulation, Plant
MicroRNAs / genetics
Passiflora* / genetics
Passiflora* / growth & development
Plant Somatic Embryogenesis Techniques* / methods
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Tissue Culture Techniques* / methods
Transformation, Genetic

Substances

MicroRNAs