Neutralizing Antibody Sample Testing and Report Harmonization

AAPS J. 2024 Jul 12;26(4):80. doi: 10.1208/s12248-024-00955-1.

Abstract

Immunogenicity testing and characterization is an important part of understanding the immune response to administration of a protein therapeutic. Neutralizing antibody (NAb) assays are used to characterize a positive anti-drug antibody (ADA) response. Harmonization of reporting of NAb assay performance and results enables efficient communication and expedient review by industry and health authorities. Herein, a cross-industry group of NAb assay experts have harmonized NAb assay reporting recommendations and provided a bioanalytical report (BAR) submission editable template developed to facilitate agency filings. This document addresses key bioanalytical reporting gaps and provides a report structure for documenting clinical NAb assay performance and results. This publication focuses on the content and presentation of the NAb sample analysis report including essential elements such as the method, critical reagents and equipment, data analysis, study samples, and results. The interpretation of immunogenicity data, including the evaluation of the impact of NAb on safety, exposure, and efficacy, is out of scope of this publication.

Keywords: bioanalytical report; immunogenicity; multi-functional therapeutics; neutralizing antibody; template.

MeSH terms

  • Antibodies, Neutralizing* / blood
  • Antibodies, Neutralizing* / immunology
  • Humans

Substances

  • Antibodies, Neutralizing