Immunohistochemical expression of SATB2 and PAX8 in differentiating primary from metastatic ovarian mucinous neoplasms

APMIS. 2024 Oct;132(10):706-717. doi: 10.1111/apm.13449. Epub 2024 Jul 15.

Abstract

Accurate stratification of an ovarian mucinous neoplasm as primary or secondary is always challenging as they show overlapping histomorphological and immunohistochemical features. Immunohistochemical staining for SATB2 and PAX8 was performed on 80 cases of mucinous ovarian neoplasms subdivided into 53 primary [25 primary ovarian mucinous carcinomas (POMCs) and 28 mucinous borderline tumors (MBTs)] and 27 secondary (12 of colonic origin, 7 of appendiceal origin, and 8 of gastric origin). Expression was correlated with different clinicopathologic parameters. PAX8-positive immunostaining was detected in 38 out of 53 cases (71.69%) of primary ovarian mucinous neoplasms (POMNs) with null positivity in the secondary ovarian mucinous tumors (0/27). SATB2-positive expression was detected in 16 out of 27 cases (59.26%) of the secondary ovarian mucinous tumors. None of the studied POMNs showed any positive immunostaining for SATB2 (0/53). A profile of SATB2-/PAX8+ and SATB2+/PAX8- can be used to differentiate POMNCs from secondary ovarian mucinous tumors of GI origin, respectively, with 100% specificity. PAX8 expression is associated with some clinicopathologic parameters providing the basis for the possible usage of PAX8 as prognostic marker.

Keywords: Ovarian cancer; PAX8; SATB2; mucinous; secondary ovarian mucinous tumors.

MeSH terms

  • Adenocarcinoma, Mucinous* / diagnosis
  • Adenocarcinoma, Mucinous* / metabolism
  • Adenocarcinoma, Mucinous* / pathology
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor* / metabolism
  • Diagnosis, Differential
  • Female
  • Humans
  • Immunohistochemistry*
  • Matrix Attachment Region Binding Proteins* / metabolism
  • Middle Aged
  • Ovarian Neoplasms* / diagnosis
  • Ovarian Neoplasms* / metabolism
  • Ovarian Neoplasms* / pathology
  • PAX8 Transcription Factor* / metabolism
  • Transcription Factors* / metabolism
  • Young Adult

Substances

  • PAX8 Transcription Factor
  • Matrix Attachment Region Binding Proteins
  • SATB2 protein, human
  • PAX8 protein, human
  • Transcription Factors
  • Biomarkers, Tumor