International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests

D M Adcock; G W Moore; G W Kershaw; S A L Montalvao; R C Gosselin

doi:10.1111/ijlh.14344

International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests

Int J Lab Hematol. 2024 Oct;46(5):777-788. doi: 10.1111/ijlh.14344. Epub 2024 Jul 15.

Authors

D M Adcock¹, G W Moore^{2

3}, G W Kershaw⁴, S A L Montalvao⁵, R C Gosselin⁶

Affiliations

¹ Retired Labcorp, Burlington, North Carolina, USA.
² Department of Haematology, Specialist Haemostasis Unit, Cambridge University Hospitals Foundation Trust, Cambridge, UK.
³ Department of Natural Sciences, Middlesex University, London, UK.
⁴ Institute of Haematology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.
⁵ Laboratory Hemostasis and Thrombosis, Hematology and Hemotherapy Center, University of Campinas UNICAMP, Campinas, Brazil.
⁶ Hemostasis and Thrombosis Center, University of California, Davis Health System, Sacramento, California, USA.

PMID: 39009028
DOI: 10.1111/ijlh.14344

Abstract

This guidance document has been prepared on behalf of the International Council for Standardization in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for the performance and interpretation of activated partial thromboplastin time (APTT) and prothrombin time (PT) plasma mixing tests in clinical laboratories in all regions of the world. The following areas are included in this document: preanalytical, analytical, postanalytical, and quality assurance considerations as they relate to the proper performance and interpretation of plasma mixing tests. The recommendations are based on good laboratory practice, published data in peer-reviewed literature, and expert opinion.

Keywords: APTT; ICSH; PT; coagulation; mixing test.

Publication types

Guideline

MeSH terms

Hematology / standards
Humans
Partial Thromboplastin Time / methods
Partial Thromboplastin Time / standards
Prothrombin Time* / standards
Reference Standards