Antifungal susceptibility pattern of Candida auris strains: Analysis of clinical strains in a tertiary-care educational university hospital

New Microbiol. 2024 Jul;47(2):152-156.

Abstract

Herein, we aimed to investigate the antifungal susceptibility pattern of Candida auris clinical strains in our setting Bahrain Oncology Center-King Hamad University Hospital-Bahrain. C. auris strains isolated from different clinical specimens in the Microbiology Laboratory from October-2021 to November-2022 were evaluated. Species-level identification of fungi was performed by MALDI-TOF (Bruker, Germany). Minimum inhibitory concentration (MIC) was determined either by E-test strips or by MICRONAUT MIC system based on CDC guidelines for C. auris antifungal interpretation. Fluconazole, amphotericin-B, voriconazole, and caspofungin susceptibility data of the clinical strains were analyzed. A total of 40 clinical isolates were included: 25% were blood culture isolates, 65% were urinary, and 10% were soft tissue isolates. Only 29 strains could be tested for amphotericin-B and 32 for voriconazole. Overall resistance pattern was as follows: 100% resistance to fluconazole, 2.5% resistance to caspofungin, and 0% resistance to amphotericin b. Median voriconazole MIC was 0.015 ug/ml (min 0.08, max= 0.064 ug/ml). We had no fluconazole-sensitive strain and only one caspofungin-resistant strain. A single isolate (2.5%), which was associated with candidemia, demonstrated resistance to two antifungal agents: fluconazole and caspofungin. No triple or quadruple drug resistant strain existed.

Keywords: Epidemiology; antifungal; candidemia; candiduria.

MeSH terms

  • Adult
  • Antifungal Agents* / pharmacology
  • Candida / drug effects
  • Candida / isolation & purification
  • Candida auris* / drug effects
  • Candidiasis* / microbiology
  • Caspofungin / pharmacology
  • Drug Resistance, Fungal*
  • Female
  • Hospitals, University*
  • Humans
  • Male
  • Microbial Sensitivity Tests*
  • Middle Aged
  • Tertiary Care Centers
  • Tertiary Healthcare
  • Voriconazole / pharmacology

Substances

  • Antifungal Agents
  • Voriconazole
  • Caspofungin