Liver and bile duct organoids and tumoroids

Xuanming Luo; Yuda Gong; Zijun Gong; Kun Fan; Tao Suo; Han Liu; Xiaoling Ni; Xiaojian Ni; Miyesaier Abudureyimu; Houbao Liu

doi:10.1016/j.biopha.2024.117104

Liver and bile duct organoids and tumoroids

Biomed Pharmacother. 2024 Sep:178:117104. doi: 10.1016/j.biopha.2024.117104. Epub 2024 Jul 17.

Authors

Xuanming Luo¹, Yuda Gong², Zijun Gong², Kun Fan³, Tao Suo², Han Liu², Xiaoling Ni², Xiaojian Ni², Miyesaier Abudureyimu⁴, Houbao Liu⁵

Affiliations

¹ Department of Biliary Surgery, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Center of Zhongshan Hospital, Fudan University, China; Cancer Center, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Institute, Fudan University, China; Shanghai Engineering Research Center of Biliary Tract Minimal Invasive Surgery and Materials, China; Department of General Surgery, Shanghai Xuhui Central Hospital, Fudan University, Shanghai, China.
² Department of Biliary Surgery, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Center of Zhongshan Hospital, Fudan University, China; Cancer Center, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Institute, Fudan University, China; Shanghai Engineering Research Center of Biliary Tract Minimal Invasive Surgery and Materials, China.
³ Department of General Surgery, Shanghai Xuhui Central Hospital, Fudan University, Shanghai, China.
⁴ Cardiovascular Department, Shanghai Xuhui Central Hospital, Fudan University, Shanghai, China. Electronic address: [email protected].
⁵ Department of Biliary Surgery, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Center of Zhongshan Hospital, Fudan University, China; Cancer Center, Zhongshan Hospital, Fudan University, China; Biliary Tract Disease Institute, Fudan University, China; Shanghai Engineering Research Center of Biliary Tract Minimal Invasive Surgery and Materials, China; Department of General Surgery, Shanghai Xuhui Central Hospital, Fudan University, Shanghai, China. Electronic address: [email protected].

PMID: 39024834
DOI: 10.1016/j.biopha.2024.117104

Abstract

Organoids refer to 3D cultures established to recapitulate histology, pathology, architecture, and genetic traits of various organs and tissues in the body, thereby replacing 2D cell cultures, xenograft, and animal models. Organoids form a 3D in vitro mimic of original tissues like the liver and are derived from embryonic or adult tissue stem cells. Liver and bile duct tumor organoids, also called, tumoroids capture genetic diversity, cellular, and pathophysiological properties of original tumors. Moreover, co-culture techniques along with genetic modulation of organoids allow for using tumoroids in liver and bile duct cancer research and drug screening/testing. Therefore, tumoroids are promising platforms for studying liver and bile duct cancer, which paves the way for the new era of personalized therapies. In the current review, we aimed to discuss liver and bile duct organoids with special emphasis on tumoroids and their applications, advantages, and shortcomings.

Keywords: Bile Duct Tumors; Embryonic and Adult Tissues; Liver Cancer; Organoids.

Publication types

Review

MeSH terms

Animals
Bile Duct Neoplasms* / genetics
Bile Duct Neoplasms* / pathology
Bile Ducts* / pathology
Humans
Liver Neoplasms* / genetics
Liver Neoplasms* / pathology
Liver* / pathology
Organoids* / pathology
Precision Medicine