Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency

Jinbin Lin; Xiangli Zheng; Jing Xia; Rongrong Xie; Jingjuan Gao; Rongrong Ye; Tingmin Liang; Mengyu Qu; Yaxin Luo; Yuemin Wang; Yuqin Ke; Chunying Li; Jinping Guo; Jianjun Lu; Weiqi Tang; Wenqing Li; Songbiao Chen

doi:10.1186/s12870-024-05391-z

Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency

BMC Plant Biol. 2024 Jul 19;24(1):689. doi: 10.1186/s12870-024-05391-z.

Authors

Jinbin Lin^#¹, Xiangli Zheng^#², Jing Xia³, Rongrong Xie^{4

5}, Jingjuan Gao^{4

5}, Rongrong Ye¹, Tingmin Liang^{1

3}, Mengyu Qu^{1

6}, Yaxin Luo¹, Yuemin Wang⁴, Yuqin Ke⁷, Chunying Li⁴, Jinping Guo⁴, Jianjun Lu¹, Weiqi Tang⁸, Wenqing Li⁹, Songbiao Chen¹⁰

Affiliations

¹ Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China.
² Fujian Key Laboratory of Agricultural Ecological Process of Red Soil Mountain, Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China.
³ College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁴ Fujian Institute of Tobacco Sciences, Fuzhou, 350003, China.
⁵ International Magnesium Institute, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁶ College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁷ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁸ Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China. [email protected].
⁹ Fujian Institute of Tobacco Sciences, Fuzhou, 350003, China. [email protected].
¹⁰ Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Geography and Oceanography, Minjiang University, Fuzhou, 350108, China. [email protected].

^# Contributed equally.

Abstract

Background: Boron (B) is an essential micronutrient for plants. Inappropriate B supply detrimentally affects the productivity of numerous crops. Understanding of the molecular responses of plants to different B supply levels would be of significance in crop improvement and cultivation practices to deal with the problem.

Results: We conducted a comprehensive analysis of the transcriptome and proteome of tobacco seedlings to investigate the expression changes of genes/proteins in response to different B supply levels, with a particular focus on B deficiency. The global gene and protein expression profiles revealed the potential mechanisms involved in the responses of tobacco to B deficiency, including up-regulation of the NIP5;1-BORs module, complex regulation of genes/proteins related to cell wall metabolism, and up-regulation of the antioxidant machinery.

Conclusion: Our results demonstrated that B deficiency caused severe morphological and physiological disorders in tobacco seedlings, and revealed dynamic expression changes of tobacco genes/proteins in response to different B supply levels, especially to B deficiency, thus offering valuable insights into the molecular responses of tobacco to B deficiency.

Keywords: Antioxidant machinery; BOR; Boron; Cell wall-related genes; NIP; Proteome analysis; Tobacco; Transcriptome analysis.

MeSH terms

Boron* / deficiency
Boron* / metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Nicotiana* / genetics
Nicotiana* / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Proteome* / metabolism
Seedlings / genetics
Seedlings / growth & development
Seedlings / metabolism
Transcriptome*

Substances

Boron
Proteome
Plant Proteins