Formononetin Derivative for Osteoporosis by Simultaneous Regulating Osteoblast and Osteoclast

J Nat Prod. 2024 Aug 23;87(8):2004-2013. doi: 10.1021/acs.jnatprod.4c00437. Epub 2024 Jul 21.

Abstract

Seven new formononetin derivatives (1-7) were designed and prepared from formononetin (phase II phytoestrogen). The derivatives 9-butyl-3-(4-methoxyphenyl)-9,10-dihydro-4H,8H-chromeno[8,7-e][1,3]oxazin-4-one (2) and 9-(furan-3-ylmethyl)-3-(4-methoxyphenyl)-9,10-dihydro-4H,8H-chromeno[8,7-e][1,3]oxazin-4-one (7) promoted significant osteoblast formation by modulating the BMP/Smad pathway. Compound 7 exhibited potent antiosteoclastogenesis activity in RANKL-induced RAW264.7 cells and ovariectomy (OVX)-induced osteoporosis in mice by regulation of the RANK/RANKL/OPG pathway. Compound 7 regulated osteoblast and osteoclast simultaneously and showed better effect than the well-known drug ipriflavone in vivo, suggesting 7 as a patented antiosteoporosis candidate.

MeSH terms

  • Animals
  • Female
  • Isoflavones* / chemistry
  • Isoflavones* / pharmacology
  • Mice
  • Molecular Structure
  • Osteoblasts* / drug effects
  • Osteoclasts* / drug effects
  • Osteoporosis* / drug therapy
  • Osteoprotegerin
  • Ovariectomy
  • RANK Ligand* / drug effects
  • RANK Ligand* / metabolism
  • RAW 264.7 Cells

Substances

  • Isoflavones
  • formononetin
  • RANK Ligand
  • ipriflavone
  • Osteoprotegerin