Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART)

Affoua-Melissa Bekon; Delphine Martiny; Anne-Laure Thomas; Yvette Miendje; Anne-Laurence Debyttere; Candice Autin; Evelyne Bertrand

doi:10.1016/j.mimet.2024.107004

Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART)

J Microbiol Methods. 2024 Sep:224:107004. doi: 10.1016/j.mimet.2024.107004. Epub 2024 Jul 22.

Authors

Affoua-Melissa Bekon¹, Delphine Martiny², Anne-Laure Thomas³, Yvette Miendje⁴, Anne-Laurence Debyttere⁴, Candice Autin³, Evelyne Bertrand⁵

Affiliations

¹ Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium; Faculté de Médecine et Pharmacie,Université de Mons (UMONS), Mons, Belgium.
² Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium; Faculté de Médecine et Pharmacie,Université de Mons (UMONS), Mons, Belgium. Electronic address: [email protected].
³ Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium.
⁴ Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium.
⁵ Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium. Electronic address: [email protected].

PMID: 39047945
DOI: 10.1016/j.mimet.2024.107004

Abstract

In ART,¹ optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.

Keywords: Assisted reproductive technology (ART); Bacteriospermia; Density gradient; Sperm preparation; Spermoculture.

MeSH terms

Cell Separation / methods
Centrifugation, Density Gradient / methods
Humans
Male
Reproductive Techniques, Assisted*
Spermatozoa*