Cordyceps cateniannulata: An endophyte of coffee, a parasite of coffee leaf rust and a pathogen of coffee pests

Fungal Biol. 2024 Aug;128(5):1917-1932. doi: 10.1016/j.funbio.2024.05.004. Epub 2024 May 29.

Abstract

Here, we report on a Cordyceps species entering into a multi-trophic, multi-kingdom association. Cordyceps cateniannulata, isolated from the stem of wild Coffea arabica in Ethiopia, is shown to function as an endophyte, a mycoparasite and an entomopathogen. A detailed polyphasic taxonomic study, including a multilocus phylogenetic analysis, confirmed its identity. An emended description of C. cateniannulata is provided herein. Previously, this species was known as a pathogen of various insect hosts in both the Old and New World. The endophytic status of C. cateniannulata was confirmed by re-isolating it from inoculated coffee plants. Inoculation studies have further shown that C. cateniannulata is a mycoparasite of Hemileia vastatrix, as well as an entomopathogen of major coffee pests; infecting and killing Hypothenemus hampei and Leucoptera coffeella. This is the first record of C. cateniannulata from Africa, as well as an endophyte and a mycoparasite. The implications for its use as a biocontrol agent are discussed.

Keywords: Biological control; Coffee berry borer; Coffee leaf miner; Host range; phylogenetics, taxonomy.

MeSH terms

  • Animals
  • Cluster Analysis
  • Coffea* / microbiology
  • Coffea* / parasitology
  • Cordyceps* / classification
  • Cordyceps* / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Endophytes* / classification
  • Endophytes* / genetics
  • Endophytes* / isolation & purification
  • Endophytes* / physiology
  • Ethiopia
  • Phylogeny*
  • Plant Diseases / microbiology
  • Plant Diseases / parasitology
  • Plant Stems / microbiology
  • Plant Stems / parasitology
  • Sequence Analysis, DNA

Substances

  • DNA, Fungal
  • DNA, Ribosomal Spacer