A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems

As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO^-) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO^- in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, SJD was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe Nano-SJD. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO^- in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO^- in real-water samples. Additionally, the robust fluorescent imaging behavior of Nano-SJD for visualization of ClO^- in living cells highlights its broad potential in biosystem applicability.