Naphthalimide-Based, Single-Chromophore, Emission Ratiometric Fluorescent Sensor for Tracking Intracellular pH

Chembiochem. 2024 Nov 4;25(21):e202400538. doi: 10.1002/cbic.202400538. Epub 2024 Oct 16.

Abstract

We report a novel, reversible, cell-permeable, pH-sensor, TRapH. TRapH afforded a pH-sensitive ratiometric emission response in the pH range ~3-6, enabling imaging and quantification of pH in living cells. The biological-applicability of TRapH was illustrated via live-tracking of intracellular pH dynamics in living mammalian cells induced by a synthetic H+-transporter.

Keywords: Emission Ratiometric pH sensor; Intracellular pH quantification; Single-dye pH sensor; Tracking Intracellular pH.

MeSH terms

  • Fluorescent Dyes* / chemical synthesis
  • Fluorescent Dyes* / chemistry
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Molecular Structure
  • Naphthalimides* / chemical synthesis
  • Naphthalimides* / chemistry
  • Optical Imaging

Substances

  • Fluorescent Dyes
  • Naphthalimides