A dual-signal mode electrochemical aptasensor based on tetrahedral DNA nanostructures for sensitive detection of citrinin in food using PtPdCo mesoporous nanozymes

Food Chem. 2024 Dec 1;460(Pt 3):140739. doi: 10.1016/j.foodchem.2024.140739. Epub 2024 Aug 3.

Abstract

Citrinin (CIT) is a mycotoxin with nephrotoxicity and hepatotoxicity, presenting a significant threat to human health that is often overlooked. Therefore, a dual-signal mode (DPV and SWV) aptasensor for citrinin (CIT) detection was constructed based on tetrahedral DNA nanostructures (TDN) in this study. Furthermore, PtPdCo mesoporous nanozymes exhibit catalase-like catalytic functions, generating significant electrochemical signals through a Fenton-like reaction. Meanwhile their excellent Methylene Blue (MB) loading capability ensures independent dual signal outputs. The RecJf exonuclease-assisted (RecJf Exo-assisted) process can expand the linear detection range, enabling further amplification of the signal. Under optimized conditions, the constructed aptaensor exhibited excellent detection performance with limits of detection (LODs) of 7.67 × 10-3 ng·mL-1 (DPV mode) and 1.57 × 10-3 ng·mL-1 (SWV mode). Due to its multiple signal amplification and highly accurate dual-signal mode detection capability, this aptasensor shows promising potential for the in situ detection.

Keywords: Dual-signal electrochemical aptasensor; Mesoporous nanozymes; Mycotoxin; Tetrahedral DNA nanostructures.

Publication types

  • Evaluation Study

MeSH terms

  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques* / instrumentation
  • Citrinin* / analysis
  • Citrinin* / chemistry
  • DNA* / chemistry
  • Electrochemical Techniques*
  • Food Contamination* / analysis
  • Limit of Detection*
  • Nanostructures* / chemistry
  • Platinum / chemistry

Substances

  • Citrinin
  • Aptamers, Nucleotide
  • DNA
  • Platinum