Characterization of an induced pluripotent stem cell line (NCHi013-A) from a 5-year-old male with pulmonary atresia with intact ventricular septum and a biventricular repair

Stem Cell Res. 2024 Oct:80:103526. doi: 10.1016/j.scr.2024.103526. Epub 2024 Jul 31.

Abstract

Pulmonary atresia with intact ventricular septum (PA/IVS) is a rare congenital heart defect that causes a significant decrease of blood outflow from the heart and is fatal if left untreated. iPSC line NCHi013-A was produced from peripheral blood mononuclear cells from a male child with PA/IVS using Sendai virus reprogramming. NCHi013-A displayed normal stem cell morphology, expressed markers for pluripotency, and presented ability to differentiate into cells of endoderm, ectoderm, and mesoderm lineages. The iPSC line also maintained normal karyotype, was validated for cell identity, and tested negative for transgenes and mycoplasma contamination.

Publication types

  • Case Reports

MeSH terms

  • Cell Differentiation
  • Cell Line
  • Child, Preschool
  • Heart Defects, Congenital / pathology
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Male
  • Pulmonary Atresia* / pathology

Supplementary concepts

  • Pulmonary Atresia with Intact Ventricular Septum