Challenges and trends in Gram-negative bacterial infections in critically neonates: A seven-and-a-half-year observational study

Am J Infect Control. 2025 Jan;53(1):13-21. doi: 10.1016/j.ajic.2024.08.004. Epub 2024 Aug 9.

Abstract

Background: Analyze the incidence, risk factors, and fatality rates of bloodstream infections by Gram-negative bacteria (GNB-BSIs) in a Neonatal Intensive Care Unit.

Methods: This study employs a retrospective cohort design utilizing records of neonates admitted to the Neonatal Intensive Care Unit between January 2015 and June 2022.

Results: Among 1,495 neonates, 5.2% developed GNB-BSIs. The average incidence of infection per 1,000 patient-days was 2.9. Primary risk factors for infection that included preceeding carbapenem use were significant risk factors (odds ratio=514.4; P < .01) and fourth-generation cephalosporins (odds ratio=66; P < .01). Among the 85 GNB, 75.3% were fermenters, and 24.7% were non-fermenters. Of the isolates, 14.1% produced extended-spectrum beta-lactamase, and 2.3% carbapenem-resistant. Infection correlated with prolonged hospital stays (10-39days) and increased mortality (10%-29.9%).

Conclusions: The high incidence of GNB-BSIs was exacerbated by the preceeding use of broad-spectrum antimicrobials, increasing the presence of multidrug-resistant isolates and fatality rates. These findings emphasize the importance of active surveillance.

Keywords: Carbapenem-resistant Enterobacteriaceae; Drug resistance; Gram-negative bacteria; Infection control; Neonate.

Publication types

  • Observational Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use
  • Bacteremia / epidemiology
  • Bacteremia / microbiology
  • Bacteremia / mortality
  • Female
  • Gram-Negative Bacteria* / drug effects
  • Gram-Negative Bacteria* / isolation & purification
  • Gram-Negative Bacterial Infections* / epidemiology
  • Gram-Negative Bacterial Infections* / microbiology
  • Humans
  • Incidence
  • Infant, Newborn
  • Intensive Care Units, Neonatal* / statistics & numerical data
  • Male
  • Retrospective Studies
  • Risk Factors

Substances

  • Anti-Bacterial Agents