Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation

Kanika Sharma; Prashant Kaushal; Vikas Kumar

doi:10.1007/978-1-0716-3969-6_10

Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation

Methods Mol Biol. 2024:2831:133-143. doi: 10.1007/978-1-0716-3969-6_10.

Authors

Kanika Sharma¹, Prashant Kaushal¹, Vikas Kumar^{2

3}

Affiliations

¹ Mass Spectrometry & Proteomics Core Facility, University of Nebraska Medical Center, Omaha, NE, USA.
² Mass Spectrometry & Proteomics Core Facility, University of Nebraska Medical Center, Omaha, NE, USA. [email protected].
³ Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA. [email protected].

PMID: 39134848
DOI: 10.1007/978-1-0716-3969-6_10

Abstract

The molecular mechanisms underlying neurite formation include multiple crosstalk between pathways such as membrane trafficking, intracellular signaling, and actin cytoskeletal rearrangement. To study the proteins involved in such complex pathways, we present a detailed workflow of the sample preparation for mass spectrometry-based proteomics and data analysis. We have also included steps to perform label-free quantification of proteins that will help researchers quantify changes in the expression levels of key regulators of neuronal morphogenesis on a global scale.

Keywords: Dendritic spine; Differentially expressed proteins; Label-free quantitative analysis; Liquid chromatography; Mass spectrometry; Neurite; Proteomics.

MeSH terms

Animals
Chromatography, Liquid / methods
Humans
Mass Spectrometry / methods
Neurites* / metabolism
Proteome / analysis
Proteome / metabolism
Proteomics* / methods

Substances

Proteome