For the genome-wide mapping of histone modifications, chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing remains the benchmark method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.
Keywords: ChIP-seq; Chromatin immunoprecipitation (ChIP); Histone modifications; Native protein-DNA interactions.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.