Fucoidan prevents diabetic cognitive dysfunction via promoting TET2-mediated active DNA demethylation in high-fat diet induced diabetic mice

Int J Biol Macromol. 2024 Oct;278(Pt 3):134186. doi: 10.1016/j.ijbiomac.2024.134186. Epub 2024 Aug 20.

Abstract

Diabetic cognitive dysfunction (DCD) refers to cognitive impairment in individuals with diabetes, which is one of the most important comorbidities and complications. Preliminary evidence suggests that consuming sufficient dietary fiber could have benefits for both diabetes and cognitive function. However, the effect and underlying mechanism of dietary fiber on DCD remain unclear. We conducted a cross-sectional analysis using data from NHANES involving 2072 diabetics and indicated a significant positive dose-response relationship between the dietary fiber intake and cognitive performance in diabetics. Furthermore, we observed disrupted cognitive function and neuronal morphology in high-fat diet induced DCD mice, both of which were effectively restored by fucoidan supplementation through alleviating DNA epigenetic metabolic disorders. Moreover, fucoidan supplementation enhanced the levels of short-chain fatty acids (SCFAs) in the cecum of diabetic mice. These SCFAs enhanced TET2 protein stability by activating phosphorylated AMPK and improved TETs activity by reducing the ratio of (succinic acid + fumaric acid)/ α-ketoglutaric acid, subsequently enhancing TET2 function. The positive correlation between dietary fiber intake and cognitive function in diabetics was supported by human and animal studies alike. Importantly, fucoidan can prevent the occurrence of DCD by promoting TET2-mediated active DNA demethylation in the cerebral cortex of diabetic mice.

Keywords: DNA demethylation; Diabetic cognitive dysfunction; Dietary fiber; Fucoidan; NHANES; TET2.

MeSH terms

  • Animals
  • Cognitive Dysfunction* / drug therapy
  • Cognitive Dysfunction* / etiology
  • Cognitive Dysfunction* / metabolism
  • Cognitive Dysfunction* / prevention & control
  • DNA Demethylation* / drug effects
  • DNA-Binding Proteins* / genetics
  • DNA-Binding Proteins* / metabolism
  • Diabetes Mellitus, Experimental* / complications
  • Diabetes Mellitus, Experimental* / metabolism
  • Diet, High-Fat* / adverse effects
  • Dietary Fiber / pharmacology
  • Dioxygenases* / metabolism
  • Fatty Acids, Volatile / metabolism
  • Female
  • Humans
  • Male
  • Mice
  • Polysaccharides* / pharmacology
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism

Substances

  • Polysaccharides
  • fucoidan
  • Dioxygenases
  • DNA-Binding Proteins
  • Tet2 protein, mouse
  • Fatty Acids, Volatile
  • Proto-Oncogene Proteins
  • Dietary Fiber