Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE-/- Mice

Cell Biochem Biophys. 2024 Sep;82(3):2297-2319. doi: 10.1007/s12013-024-01341-6. Epub 2024 Aug 22.

Abstract

This study established an LPS-induced RAW264.7 macrophage inflammatory injury model and an AS mouse vulnerable plaque model to observe the effect of JPHYP on macrophage inflammation, plaque formation, blood lipids, inflammation levels, intestinal flora and the influence of TLR4/MyD88/MAPK pathway, and explore the anti-AS effect and molecular mechanism of JPHYP, and detected 16S rRNA of mice intestinal microbes. The difference of intestinal flora in different groups of mice was compared to further explore the intervention effect of JPHYP and clarify the molecular biological mechanism of JPHYP in preventing and treating AS by regulating TLR4/MyD88/MAPK inflammatory signaling pathway and improving intestinal flora.

Keywords: Atherosclerosis; Inflammation; Intestinal flora; Jianpi Huayu Prescription; TLR4/MyD88/MAPK signal path.

MeSH terms

  • Animals
  • Apolipoproteins E* / deficiency
  • Apolipoproteins E* / genetics
  • Atherosclerosis* / drug therapy
  • Atherosclerosis* / pathology
  • Atherosclerosis* / prevention & control
  • Disease Models, Animal
  • Drugs, Chinese Herbal* / pharmacology
  • Drugs, Chinese Herbal* / therapeutic use
  • Gastrointestinal Microbiome* / drug effects
  • Inflammation*
  • Lipopolysaccharides
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Knockout
  • Myeloid Differentiation Factor 88* / metabolism
  • RAW 264.7 Cells
  • Signal Transduction / drug effects
  • Toll-Like Receptor 4* / metabolism

Substances

  • Toll-Like Receptor 4
  • Myeloid Differentiation Factor 88
  • Apolipoproteins E
  • Drugs, Chinese Herbal
  • Lipopolysaccharides
  • Myd88 protein, mouse
  • Tlr4 protein, mouse