New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish

Karolina Ucieklak; Sylwia Wojtys-Tekiel; Garance Leroy; Laëtitia Le Devendec; Sandrine Baron; Marta Kaszowska

doi:10.3390/microorganisms12081575

New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish

Microorganisms. 2024 Aug 1;12(8):1575. doi: 10.3390/microorganisms12081575.

Authors

Karolina Ucieklak¹, Sylwia Wojtys-Tekiel¹, Garance Leroy², Laëtitia Le Devendec³, Sandrine Baron³, Marta Kaszowska¹

Affiliations

¹ Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
² Laboratory of Marine Biotechnology and Chemistry, University of Western Brittany, EMR CNRS 6076, IUEM, 29000 Quimper, France.
³ Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit Ploufragan Plouzane-Niort Laboratory (ANSES), 22440 Ploufragan, France.

Abstract

The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by ¹H and ¹³C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established: →3)-α-L-Rhap-(1→3)-α-D-ManpNAc-(1→2)-β-D-Glcp-(1→3)-α-L-Rhap2OAc4OAc-(1→3)-β-D-ManpNAc-(1→3)-α-D-Glcp-(1→. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish.

Keywords: Aeromonas salmonicida; HR MAS NMR; O-polysaccharide diversity; lipopolysaccharide.

Grants and funding

This study was financed through the «ANTIBIOFISH» research project supported by both French ministries in charge of environment and agriculture. This project is part of the national effort to reduce antimicrobial resistance in veterinary medicine called “EcoAntibio2017”. This work was supported by the Polish National Agency for Academic Exchange (NAWA), mobility grant number PPN/BFR/2020/1/00031 and 46907VD-PHC Polonium 2021. This work was supported by the statutory funds of the Laboratory of Microbial Immunochemistry and Vaccines of the Hirszfeld Institute of Immunology & Experimental Therapy, Polish Academy of Sciences.